SOX2 Antibody

• Background:

  SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in this gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation. This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT) . Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

• NCBI Gene ID:

  6657

• Swiss Prot:

  P48431

• Accession Number:

  NP_003097

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SOX2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  OVCAR-3 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SOX2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  34 kDa

• Applications Notes:

  SOX2 antibody can be used for detection of SOX2 by ELISA at 1:12500. SOX2 antibody can be used for detection of SOX2 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  SOX2

• NCBI Official Name:

  SRY (sex determining region Y) -box 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wang, P., (2008) Arch. Ophthalmol. 126 (5), 709-713 .

• Other Product Names:

  SOX2, ANOP3, MCOPS3, MGC2413

• Tested Applications:

  ELISA, WB

• Protein ID:

  28195386

• Physical Properties:

  Liquid