RELA Antibody

• Background:

  P65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.

• NCBI Gene ID:

  5970

• Swiss Prot:

  Q04206

• Accession Number:

  NP_068810

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RELA.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer, Signal Transduction, Immunology, Apoptosis

• Purification:

  Antibody is purified by Protein A/G affinity chromatography method.

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store RELA antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  60 kDa

• Applications Notes:

  RELA antibody can be used for detection of RELA by ELISA at 1:62500. RELA antibody can be used for detection of RELA by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  RELA

• NCBI Official Name:

  V-rel avian reticuloendotheliosis viral oncogene homolog A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ruben, S.M., et al., (1991) Science 251 (5000) 1490-1493..

• Other Product Names:

  RELA, p65, NFKB3

• Tested Applications:

  ELISA, WB

• Protein ID:

  223468676

• Physical Properties:

  Liquid