NANOG Antibody

• Background:

  NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.

• NCBI Gene ID:

  79923

• Swiss Prot:

  Q6NSW7

• Accession Number:

  NP_079141

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NANOG.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NANOG antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  35 kDa

• Applications Notes:

  NANOG antibody can be used for detection of NANOG by ELISA at 1:62500. NANOG antibody can be used for detection of NANOG by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NANOG

• NCBI Official Name:

  Nanog homeobox

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Piestun, D., (2006) Biochem. Biophys. Res. Commun. 343 (1), 279-285.

• Other Product Names:

  NANOG

• Tested Applications:

  ELISA, WB

• Protein ID:

  153945816

• Physical Properties:

  Liquid