NKX2-8 Antibody

• Background:

  A murine NKX2-8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. Nkx2.8 bound to the active AFP promoter, and antisense inhibition of Nkx2.8 mRNA translation selectively reduced expression of both the endogenous human AFP gene and transfected reporters containing the rat AFP promoter.

• NCBI Gene ID:

  26257

• Swiss Prot:

  O15522

• Accession Number:

  NP_055175

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NKX2-8.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NKX2-8 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  26 kDa

• Applications Notes:

  NKX2-8 antibody can be used for detection of NKX2-8 by ELISA at 1:12500. NKX2-8 antibody can be used for detection of NKX2-8 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NKX2-8

• NCBI Official Name:

  NK2 homeobox 8

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hamamoto, R., (2004) Nat. Cell Biol. 6 (8), 731-740.

• Other Product Names:

  NKX2-8, NKX2.8, NKX2H, Nkx2-9

• Tested Applications:

  ELISA, WB

• Protein ID:

  31377777

• Physical Properties:

  Liquid