GTF2A1 Antibody

• Background:

  GTF2A1 belongs to the TFIIA subunit 1 family. It is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. This protein induces a conformational change within the TBP/TATA complex that enhances its stability under both in vitro and physiological salt conditions.

• NCBI Gene ID:

  2957

• Swiss Prot:

  P52655

• Accession Number:

  NP_056943

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2A1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GTF2A1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  41 kDa

• Applications Notes:

  GTF2A1 antibody can be used for detection of GTF2A1 by ELISA at 1:62500. GTF2A1 antibody can be used for detection of GTF2A1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  GTF2A1

• NCBI Official Name:

  General transcription factor IIA, 1, 19/37kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hieb, A.R., (2007) J. Mol. Biol. 372 (3), 619-632.

• Other Product Names:

  GTF2A1, MGC129969, MGC129970, TF2A1, TFIIA, TFIIAL, TFIIA-42

• Tested Applications:

  ELISA, WB

• Protein ID:

  7706735

• Physical Properties:

  Liquid