SAMHD1 Antibody

• Background:

  The SAM domain and HD domain 1 (SAMHD1) protein is upregulated in response to viral infection and is thought to play a role in innate immunity (1) . SAMHD1 blocks the infection of HIV-1 and SIVdeltaVpx before reverse transcription in macrophages and dendritic cells (2), and this restriction is regulated by phosphorylation of SAMHD1 (3) . Mutations in this gene have been associated with Aicardi-Goutieres syndrome (1) .

• NCBI Gene ID:

  25939

• Swiss Prot:

  Q9Y3Z3

• Accession Number:

  NP_056289

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  8007

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  KO Validation (Figure 5) shows SAMHD1 expression detected by anti-SAMHD1 antibodies (8007) was disrupted in multiple cells of SAMHD1 KO mice (figure 6) . KO Validation (8007) Overexpression validation (Figure 2,6,7) : SAMHD1 overexpression detected by anit-SAMHD1 antibodies (8007) was observed in normal cell lines stably across all healthy donors. Overexpression validation8007 Regulated expression validation (Figure 8 & 9) : SAMHD1 expression detected by anit-SAMHD1 antibodies (8007) was down-regulated by nucleofection with miR-181b at 48hr (figure 8), but up-regulated by treatment of miR-181family inhibitor (figure 9) . Regulated expression validation8007

• Field of Research:

  Innate Immunity

• Purification:

  SAMHD1 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1224 - Daudi Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  SAMHD1 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  SAMHD1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  SAMHD1 antibody is human and mouse reactive.

• Isoform:

  Human SAMHD1 has 3 isoforms, including isoform 1 (626aa, 72kD), isoform 3 (556aa, 64kD), isoform 4 (591aa, 68kD) . Mouse SAMHD1 has 2 isoforms, including (658aa, 76kD) and (651aa, 75kD) . Rat SAMHD1 has only one isoform (620aa, 72kD) . 8007 can detect human, mouse and rat.

• Symbol:

  SAMHD1

• NCBI Official Name:

  SAM domain and HD domain 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rice et al. Nat. Genet. 2009; 41:829-32.

• Background Reference 02:

  Hrecka et al. Nature 2011; 474:654-7.

• Background Reference 03:

  Welbourn et al. J. Virol. 2013; 87:11516-24.

• Citation 01:

  29911928

• Citation 02:

  27889686

• Citation 03:

  29321329

• Citation 04:

  26580513

• Other Product Names:

  SAM domain and HD domain 1, DCIP, CHBL2, HDDC1, MOP-5, SBBI88

• Tested Applications:

  ELISA, WB, IHC, IF

• Protein ID:

  38016914

• Physical Properties:

  Liquid