ITCH Antibody

• Background :

  The Itchy E3 ubiquitin protein ligase (ITCH) is a member of the Nedd4 family of HECT domain E3 ubiquitin ligases (1) . HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. ITCH plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses (2) . In B cells, ITCH is thought to associate with latent membrane protein 2A (LMP2A) of Epstein-Barr virus, specifically down-regulating its activity in B cell signaling (3) . Mutations in this gene are a cause of syndromic multisystem autoimmune disease (4) .

• NCBI Gene ID :

  83737

• Swiss Prot :

  Q96J02

• Accession Number :

  NP_001244066

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Stem Cell

• Purification :

  ITCH antibody is affinity chromatography purified via peptide column.

• Positive Control :

  Cat. No. 1282 - 3T3 (NIH) Cell Lysate

• Concentration :

  1 mg/mL

• Buffer :

  ITCH antibody is supplied in PBS containing 0.02% sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  ITCH antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment :

  IgG

• Specificity :

  ITCH antibody is human, mouse and rat reactive. At least three isoforms of ITCH are known to exist; this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.

• Symbol :

  ITCH

• NCBI Official Name :

  Itchy E3 ubiquitin protein ligase

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Perry WL, Hustad CM, Swing DA, et al. The itchy locus encodes a novel ubiquitin protein ligase that is disrupted in a18H mice. Nat. Genet. 1998; 18:143-6.

• Background Reference 02 :

  Melino G, Gallagher E, Aqeilan RI, et al. Itch: a HECT-type E3 ligase regulating immunity, skin and cancer. Cell Death Differ. 2008; 15:1103-12.

• Background Reference 03 :

  Ikeda A, Caldwell RG, Longnecker R, et al. Itchy, a Nedd4 ubiquitin ligase, downregulates latent membrane protein 2A activity in B-cell signaling. J. Virol. 2003; 77:5529-34.

• Background Reference 04 :

  Matesic LE, Copeland NG, and Jenkins NA. Itchy mice: the identification of a new pathway for the development of autoimmunity. Curr. Top. Microbiol. Immunol. 2008; 321:185-200.

• Other Product Names :

  ITCH Antibody: AIF4, AIP4, NAPP1, dJ468O1.1, E3 ubiquitin-protein ligase Itchy homolog, Atrophin-1-interacting protein 4, Itch

• Tested Applications :

  ELISA, WB

• Protein ID :

  380420335

• Physical Properties :

  Liquid