RNF20 Antibody

• Background :

  RNF20 Antibody: RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS) . In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.

• NCBI Gene ID :

  56254

• Swiss Prot :

  Q5VTR2

• Accession Number :

  NP_062538

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Stem Cell

• Purification :

  RNF20 Antibody is affinity chromatography purified via peptide column.

• Positive Control :

  Cat. No. 1304 - Human Liver Tissue Lysate

• Concentration :

  1 mg/mL

• Homology :

  Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (89%)

• Buffer :

  RNF20 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  RNF20 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment :

  IgG

• Specificity :

  Multiple isoforms of RNF20 are known to exist.

• Symbol :

  RNF20

• Positive Control 2 :

  Cat. No. 10-201 - Human Liver Tissue Slide

• NCBI Official Name :

  Ring finger protein 20, E3 ubiquitin protein ligase

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Zhu B, Zheng Y, Pham AD, et al. Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation. Mol. Cell 2005; 20:601-11.

• Background Reference 02 :

  Shema E, Kim J, Roeder RG, et al. RNF20 inhibits TFIIS-facilitated transcriptional elongation to suppress pro-oncogenic gene expression. Mol Cell 2011; 42:477-88.

• Background Reference 03 :

  Shema E, Tirosh I, Aylon Y, et al. The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression. Genes Dev. 2008; 22:2664-76.

• Background Reference 04 :

  Fuchs G, Shema E, Vesterman R, et al. RNF20 and USP44 regulate stem cell differentiation by modulating H2B monoubiquitylation. Mol. Cell 2012; 46:662-73.

• Other Product Names :

  RNF20 Antibody: BRE1, BRE1A, hBRE1, E3 ubiquitin-protein ligase BRE1A, RING finger protein 20, BRE1-A

• Tested Applications :

  ELISA, WB, IHC-P, IF

• Protein ID :

  34878777

• Physical Properties :

  Liquid