Alpha-tubulin Antibody

• Background:

  Alpha-tubulin Antibody: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.

• NCBI Gene ID:

  7278

• Swiss Prot:

  Q13748

• Accession Number:

  NP_005992

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  Alpha-tubulin Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1403 - Mouse Brain Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%), Chicken: (100%)

• Buffer:

  Alpha-tubulin Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Alpha-tubulin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Calculated Molecular Weight:

  Predicted: 50 kDa

• Fragment:

  IgG

• Symbol:

  TUBA3C

• NCBI Official Name:

  Tubulin, alpha 3c

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33.

• Background Reference 02:

  Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 1988; 2:133-53.

• Background Reference 03:

  Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem. 2009; 109:683-693.

• Other Product Names:

  Alpha-tubulin Antibody: TUBA2, bA408E5.3, TUBA2, TUBA3D, Alpha-tubulin 2

• Tested Applications:

  ELISA, WB

• Protein ID:

  17921993

• Physical Properties:

  Liquid