PHOX2B Antibody

• Background:

  PHOX2B Antibody: PHOX2A and PHOX2B (Paired mesoderm homeobox protein) are closely related, paired-homeodomain transcription factors that function as determinants of the noradrenergic phenotype during embryogenesis. PHOX2 proteins are crucial for the regulation of endogenous hydroxylases in neural crest cells and promote sympathetic neuron generation. Human PHOX2B contains one DNA binding homeobox domain and is required for the differentiation of all central and nonperipheral noradrenergic centers in the brain. In contrast, PHOX2A controls only the differentiation of the main noradrenergic center of the brain. Regulation of PHOX2 may have therapeutic utility in aging or disorders involving degeneration of noradrenergic neurons.

• NCBI Gene ID:

  8929

• Swiss Prot:

  Q99453

• Accession Number:

  NP_003915

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  PHOX2B Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1210 - HEK293 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Chicken: (75%), Bovine: (79%)

• Buffer:

  PHOX2B Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PHOX2B antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  PHOX2B antibody is predicted to not cross-react with other paired homeobox family members.

• Symbol:

  PHOX2B

• NCBI Official Name:

  Paired-like homeobox 2b

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Stanke M, Junghans D, Geissen M, et al. The Phox2 homeodomain proteins are sufficient to promote the development of sympathetic neurons. Development 1999; 126:4087-94

• Background Reference 02:

  Young HM, Ciampoli D, Hsuan J, et al. Expression of Ret-, p75 (NTR) -, Phox2a-, Phox2b-, and tyrosine hydroxalase-immunoreactivity by undifferentiated neural crest-derived cells and different classes of enteric neurons in the embryonic mouse gut. Dev. Dyn. 1999; 216:137-52.

• Background Reference 03:

  Pattyn A, Morin X, Cremer H, et al. The homeobox gene Phox2b is essential for the development of autonomic neural crest derivatives. Nature 1999; 399:366-70.

• Background Reference 04:

  Coppola E, d'Autréaux F, Rijli FM, et al. Ongoing roles of Phox2 homeodomain transcription factors during neuronal differentiation. Development 2010; 137:4211-20

• Other Product Names:

  PHOX2B Antibody: PMX2B, NBLST2, NBPhox, PMX2B, Paired mesoderm homeobox protein 2B, Neuroblastoma Phox

• Tested Applications:

  ELISA, WB

• Protein ID:

  12707580

• Physical Properties:

  Liquid