RANBP10 Antibody

• Background:

  RANBP10 Antibody: The RAN binding protein 10 (RANBP10) is a cytoplasmic guanine nucleotide exchange factor that shares high sequence similarity to the MET-interacting protein RANBP9. RANBP10 is expressed in the cytoplasm of mature megakaryocytes (MKs) and platelets where it localizes to polymerized noncentrosomal microtubules. RANBP10 possesses guanine nucleotide exchange factor activity towards RAN, a GTPase involved in nucleocytoplasmic traffic, spindle formation at mitosis, and nuclear envelope assembly during telophase, suggesting that RANBP10 may also be involved in these activities. RANBP10 depletion in vitro caused the disturbance of polymerized filaments in MKs and platelets in RANBP10-null mice exhibited disorders in microtubule filament numbers and localization, indicating that RANBP10 may play a significant role in maintaining microtubule dynamics.

• NCBI Gene ID:

  57610

• Swiss Prot:

  Q6VN20

• Accession Number:

  EAW83167

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  RANBP10 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (93%), Mouse: (87%)

• Buffer:

  RANBP10 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  RANBP10 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  RANBP10

• NCBI Official Name:

  RAN binding protein 10

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wang D, Zaibo Li, Schoen SR, et al. A novel MET-interacting protein shares high sequence similarity with RanBPM, but fails to stimulate MET-induced Ras/ERK signaling. Biochem. Biophys. Res. Comm.2004; 313:320-6.

• Background Reference 02:

  Schulze H, Dose M, Korpal M, et al. RanBP10 is a cytoplasmic guanine nucleotide exchange factor that modulates noncentrosomal microtubules. J. Biol. Chem.2008; 283:14109-19.

• Background Reference 03:

  Dasso M. The Ran GTPase: theme and variations. Curr. Biol.2002; R502-8.

• Background Reference 04:

  Kunert S, Meyer I, Fleischhauer S, et al. The microtubule modulator RanBP10 plays a critical role in regulation of platelet discoid shape and degranulation. Blood2009; 114:5532-40.

• Other Product Names:

  RANBP10 Antibody: KIAA1464, Ran-binding protein 10, RanBP10

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  119603573

• Physical Properties:

  Liquid