KLF4 Antibody

• Background:

  KLF4 Antibody: KLF4 is a transcription factor that functions as both a transcriptional activator and repressor to regulate proliferation and differentiation of multiple cell types. The role of KLF4 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing KLF4 and the transcription factors POU5F1, Sox2, and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing KLF4, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. KLF4 interacts directly with POU5F1 and Sox2 in iPS and ES cells and activates the target gene NANOG.

• NCBI Gene ID:

  9314

• Swiss Prot:

  O43474

• Accession Number:

  AAH30811

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Relative Expression Validation (Figure 1) : Anti-KLF4 antibodies (5603) specificity was further verified by KLF4 relative expression. KLF4 protein was detected in MCF-7 cells, but not in Jurkat cells, which matches the RNA expression pattern in these two cell lines based on Human Protein Atlas data (https://www.proteinatlas.org/ENSG00000136826-KLF4/subcellular) . Relative Expression Validation5603) KLF4 protein was detected in MCF-7 cells, but not in Jurkat cells, which matches the RNA expression pattern in these two cell lines based on Human Protein Atlas data (https://www.proteinatlas.org/ENSG00000136826-KLF4/subcellular

• Field of Research:

  Stem Cell

• Purification:

  KLF4 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1219 – MCF-7 cell lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Pig: (80%), Bovine: (74%), Chicken: (75%)

• Buffer:

  KLF4 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  KLF4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human KLF4 has 5 isoforms including isoform 1 (513aa, 55kD), isoform 2 (479aa, 51kDa), isoform 3 (429aa, 46kDa), isoform 4 (118aa, 12kDa), and isoform 5 (63aa, 6.5kDa) ; Mouse KLF4 has one isoform (483aa, 52kD and rat KLF4 has one isoform (482aa, 52kD) .

• Symbol:

  KLF4

• NCBI Official Name:

  Kruppel-like factor 4 (gut)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Evans PM, Zhang W, Chen X, et al. Kruppel-like factor 4 is acetylated by p300 and regulates gene transcription via modulation of histone acetylation. J. Bio. Chem.2007; 282:33994-4002.

• Background Reference 02:

  Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.

• Background Reference 03:

  Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell2006; 1263:663-76.

• Background Reference 04:

  Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science2008; 322:949-53.

• Other Product Names:

  KLF4 Antibody: EZF, GKLF, EZF, Krueppel-like factor 4, Epithelial zinc finger protein EZF

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  21410813

• Physical Properties:

  Liquid