NANOG Antibody

• Background:

  NANOG Antibody: Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

• NCBI Gene ID:

  79923

• Swiss Prot:

  Q9H9S0

• Accession Number:

  EAW88651

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis, Stem Cell

• Purification:

  NANOG Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1416 – Mouse Testis Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (89%), Chicken: (94%)

• Buffer:

  NANOG Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  NANOG antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  Predicted: 35 kD Observed: 35 kD

• Fragment:

  IgG

• Isoform:

  Human NANOG has 2 isoforms, isoform 1 (305aa, 34.6kD) and isoform 2 (289aa, 32.8kDa) . Mouse NANOG has 2 isoforms, isoform 1 (305aa, 34.2kD) and isoform 2 (280aa, 31.9kD) . Rat NANOG has 1 isoform (311aa, 35.1kD) . 5585 can detect NANOG in human, mouse, and rat.

• Symbol:

  NANOG

• Positive Control 2:

  Cat. No. 1476 – Rat Testis Tissue Lysate

• NCBI Official Name:

  Nanog homeobox

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chambers I, Colby D, Robertson M, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell2003; 113:643-55.

• Background Reference 02:

  Mitsui K, Tokuzowa Y, Itoh H, et al. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell2003; 113:631-42.

• Background Reference 03:

  Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.

• Background Reference 04:

  Scholer HR, Ruppert S, Suzuki N, et al. New type of POU domain in germ line-specific protein Oct-2. Nature1990; 344:435-9.

• Other Product Names:

  NANOG Antibody: Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog

• Tested Applications:

  ELISA, WB, ICC/IF, IHC-P

• Protein ID:

  119609057

• Physical Properties:

  Liquid