SOX2 Antibody

• Background :

  SOX2 Antibody: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. SOX2 is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. The role of SOX2 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing SOX2 and the transcription factors POU5F1, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Other experiments have shown that iPS cells could be generated using expression plasmids expressing POU5F1, SOX2, KlfF4 and c-Myc, eliminating the need for virus introduction.

• NCBI Gene ID :

  6657

• Swiss Prot :

  P48431

• Accession Number :

  NP_003097

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Homeostasis, Stem Cell

• Purification :

  SOX2 Antibody is affinity chromatography purified via peptide column.

• Positive Control :

  Cat. No. 1282 - 3T3 (NIH) Cell Lysate

• Concentration :

  1 mg/mL

• Homology :

  Predicted species reactivity based on immunogen sequence: Sheep: (100%), Chicken: (100%), Horse: (83%), Pig: (100%)

• Buffer :

  SOX2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  SOX2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment :

  IgG

• Symbol :

  SOX2

• Positive Control 2 :

  Cat. No. 17-201 - 3T3/BALB Cell Slide

• NCBI Official Name :

  SRY (sex determining region Y) -box 2

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Hever AM, Williamson KA, and van Heyningen V. Developmental malformations of the eye: the role of PAX6, SOX2 and OTX2. Clin. Genet.2006; 69:459-70.

• Background Reference 02 :

  Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells2003; 5:79-88.

• Background Reference 03 :

  Takahashi K and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell2006; 1263:663-76.

• Background Reference 04 :

  Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science2008; 322:949-53.

• Other Product Names :

  SOX2 Antibody: ANOP3, MCOPS3, Transcription factor SOX-2

• Tested Applications :

  ELISA, WB, ICC, IHC-P, IF

• Protein ID :

  28195386

• Physical Properties :

  Liquid