ATG16 Antibody

• Background:

  ATG16 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.

• NCBI Gene ID:

  55054

• Swiss Prot:

  Q676U5

• Accession Number:

  NP_110430

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Autophagy

• Purification:

  ATG16 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (94%)

• Buffer:

  ATG16 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  ATG16 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  ATG16L1

• Positive Control 2:

  Cat. No. 17-001 - HeLa Cell Slide

• NCBI Official Name:

  ATG16 autophagy related 16-like 1 (S. cerevisiae)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.

• Background Reference 02:

  Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis1993; 14:2501-5.

• Background Reference 03:

  Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol.2000; 150:1507-13.

• Background Reference 04:

  Mizushima N, Noda T, and Ohsumi Y. Apg16p is required for the function of the apg12p-apg5p conjugate in the yeast autophagy pathway. EMBO J.1999; 18:3888-96.

• Other Product Names:

  ATG16 Antibody: IBD10, WDR30, APG16L, ATG16A, ATG16L, UNQ9393/PRO34307, Autophagy-related protein 16-1, APG16-like 1

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  124256480

• Physical Properties:

  Liquid