TRIM25 Antibody

• Background:

  TRIM25 Antibody: TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.

• NCBI Gene ID:

  7706

• Swiss Prot:

  Q14258

• Accession Number:

  NP_005073

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  TRIM25 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (80%)

• Buffer:

  TRIM25 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TRIM25 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  TRIM25

• Positive Control 2:

  Cat. No. 17-001 - HeLa Cell Slide

• NCBI Official Name:

  Tripartite motif-containing 25

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Inoue S, Orimo A, Hosoi T, et al. Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein. Proc. Natl. Acad. Sci. USA1993; 11117-20.

• Background Reference 02:

  Orimo A, Inoue S, Minowa O, et al. Underdeveloped uterus and reduced estrogen responsiveness in mice with disruption of the estrogen-responsive finger protein gene, which is a direct target of estrogen receptor a. Proc. Natl. Acad. Sci. USA1999; 96:12027-32.

• Background Reference 03:

  Thomson SD, Ali S, Pickles L, et al. Analysis of estrogen-responsive finger protein expression in benign and malignant human breast. Int. J. Cancer2001; 91:152-8.

• Background Reference 04:

  Urano T, Saito T, Tsukui T, et al. Efp targets 14-3-3s for proteolysis and promotes breast tumour growth. Nature2002; 417:871-5.

• Other Product Names:

  TRIM25 Antibody: EFP, Z147, RNF147, ZNF147, EFP, Estrogen-responsive finger protein

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  68160937

• Physical Properties:

  Liquid