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IL-33 Antibody

CAT: 0223-4273-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-4273-01Size:0.02 mg
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Background
IL-33 Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1α /beta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP) . Receptor binding of IL-33 activates NF-κ B and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.
NCBI Gene ID
90865
Swiss Prot
O95760
Accession Number
NP_254274
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
4273
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Recombinant Protein Validation (Figure 2) shows expression of human partial recombinant protein IL-33 detected by anti-IL-33 antibody, 4273. Bands of 18kD (monomer) and 36kD (dimer) were observed. Recombinant Protein Validation 4273 Regulated Expression Validation (Figure 7&8) shows the up-regulation (Figure 7) of IL-33 detected by anti-IL-33 antibody in human skin fibroblasts after α-irradiation and this effect was inhibited by PPP (Figure 8) . Regulated Expression Validation
Field of Research
Immunology
Purification
IL-33 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1288 - A20 Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Rat: (76.5%)
Buffer
IL-33 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
IL-33 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human IL-33 has 4 isoforms, including isoform 1 (270aa, 30.8kD), isoform 2 (228aa, 25.9kD), isoform 3 (228aa, 26.1kD) and isoform 4 (144aa, 16.3kD) . This antibody detects only human isoform L. Mouse IL-33 has 1 isoform (266aa, 30kD) . Rat IL-33 has only one isoform identified so far (264aa, 29.5kD) .
Symbol
IL33
Positive Control 2
Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
NCBI Official Name
Interleukin 33
NCBI Organism
Homo sapiens
Background Reference 01
Schmitz J et al. Immunity 2005; 23:479-90.
Background Reference 02
Dinarello Eur. Cytokine Netw. 2000; 11:483-6.
Background Reference 03
Brint et al. Nat. Immunol. 2004; 5:373-9.
Background Reference 04
Chackerian et al. J. Immunol. 2007; 179:2551-5.
Citation 01
20206688
Citation 02
22785132
Other Product Names
IL-33 Antibody: DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26, NFHEV, Interleukin-33, Interleukin-1 family member 11, IL-33
Tested Applications
ELISA, WB, IF, IHC-P
Protein ID
15559209
Physical Properties
Liquid

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