TSLP Antibody

• Background:

  TSLP Antibody: Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. At least two differentially spliced isoforms of TSLP are known to exist.

• NCBI Gene ID:

  85480

• Swiss Prot:

  Q969D9

• Accession Number:

  NP_149024

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  4021

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Recombinant Protein Validation (Figure 1) : Anit-TSLP antibodies (4021) detected human TSLP recombinant protein at different concentrations. Recombinant Protein Validation4021 Regulated Expression Validation (Figure 4-8) : Anit-TSLP antibodies (4021) detected increased expression in multiple tissue or cells after treatment with cytokines or chemicals, in which the increase in the expression was inhibited by co-treatment with anti-cholinergic drugs (Figure 5) . Regulated Expression Validation4021

• Field of Research:

  Immunology

• Purification:

  TSLP Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  TSLP Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  TSLP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human TSLP has 2 isoforms, including isoform 1 (159aa, 18kD) and isoform 2 (63aa, 7kD) . Mouse TSLP has one isoform (140aa, 16kD) and rat TSLP also has one isoform (135aa, 16kD) . 4021 can detect only human isoforms, but cannot detect mouse and rat TSLP.

• Symbol:

  TSLP

• Positive Control 2:

  Cat. No. 1303 - Human Brain Tissue Lysate

• Positive Control 3:

  Cat. No. 10-301 - Human Brain Tissue Slide

• NCBI Official Name:

  Thymic stromal lymphopoietin

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ziegler and Liu. Nature Immunol. 2006; 7:709-14.

• Background Reference 02:

  Sims et al. J. Exp. Med. 2000; 192:671-80.

• Background Reference 03:

  Levin et al. J. Immunol. 1999; 162:677-83.

• Background Reference 04:

  Quentmeier et al. Leukemia 2001; 15:1286-92.

• Citation 01:

  PMID: 24850429.

• Citation 02:

  PMID: 30291224.

• Citation 03:

  PMID: 29803543.

• Citation 04:

  PMID: 23550226.

• Citation 05:

  PMID: 24381572.

• Citation 06:

  PMID: 21820713.

• Citation 07:

  PMID: 20107175.

• Citation 08:

  PMID: 19151401.

• Other Product Names:

  TSLP Antibody: Thymic stromal lymphopoietin

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  14719428

• Physical Properties:

  Liquid