Lipe Antibody

• Background:

  Lipe Antibody: Although initially described as an adipocyte-specific triacylglycerol lipase, Lipe (also known as hormone-specific lipase or HSL) is expressed in multiple tissues and cell lines. It plays multiple roles in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. Lipe is expressed as a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesterol esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Recently, it was observed that the lack of Lipe in genetically obese leptin-null mice inhibited obesity and adipogenesis, suggesting that Lipe plays a major role in adipocyte proliferation.

• NCBI Gene ID:

  3991

• Swiss Prot:

  Q05469

• Accession Number:

  ABA03168

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity

• Purification:

  Lipe Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1369 - Human Lymph node Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (93%)

• Buffer:

  Lipe Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Lipe antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  Multiple isoforms of Lipe are known to exist.

• Symbol:

  LIPE

• Positive Control 2:

  Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide

• NCBI Official Name:

  Lipase, hormone-sensitive

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yeaman SJ. Hormone-sensitive lipase - new roles for an old enzyme. Biochem. J. 2004; 379:11-22.

• Background Reference 02:

  Holst LS, Langin D, Mulder H, et al. Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase. Genomics 1996; 35:441-7.

• Background Reference 03:

  Kraemer FB, Shen WJ, Harada K, et al. Hormone-sensitive lipase is required for high-density lipoprotein cholesterol ester-supported adrenal steroidogenesis. Mol. Endocrinol. 2004; 18:549-57.

• Background Reference 04:

  Sekiya M, Osuga J, Okazaki H, et al. Absence of hormone-sensitive lipase inhibits obesity and adipogenesis in Lep ob/ob mice. J. Biol. Chem.2004; 279:15084-90.

• Other Product Names:

  Lipe Antibody: HSL, LHS, Hormone-sensitive lipase, HSL

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  896474

• Physical Properties:

  Liquid