SRANK Ligand Antibody

• Background:

  SRANK Ligand Antibody: The receptor activator of NF-κ B ligand (RANK-L) is a recently discovered member of the TNF-ligand family involved in the regulation of the T cell-dependent immune response, lymph node organogenesis and bone formation. RANK-L exists as both a normal, transmembrane form and a truncated, soluble form (sRANK-L), both of which can stimulate the receptor. Activation of T cells, such as by treatment with interleukin-7, induces RANK-L production and leads to an increase of osteoclast formation and bone loss. Finally, sRANK-L can activate the antiapoptotic kinase Akt through a signaling complex involving Src kinase and TRAF6, suggesting sRANK-L may also play a role in regulating apoptosis. This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.

• NCBI Gene ID:

  8600

• Swiss Prot:

  O14788

• Accession Number:

  NP_003692

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  3963

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Recombinant Protein Test (Figure 3) : Anti-sRANK-L antibodies (3963) detected human sRANK-L recombinant protein at different concentrations. Recombinant Protein Test3963 Induced expression validation (Figure 6) : sRANK-L expression detected by anit-sRANK-L antibodies (3963) was up-regulated after 18hr by extraction of upper jaw molars in rat osteogenic cells. Induced expression validation3963

• Field of Research:

  Apoptosis

• Purification:

  SRANK Ligand Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1464 - Rat Liver Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  SRANK Ligand Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  SRANK Ligand antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human sRANK-L has 3 isoforms, including isoform 1 (317aa, 35.5kD), isoform 2 (244aa, 27.7kD) and isoform 3 (270aa, 30.5kD) . Mouse sRANK-L also has 3 isoforms, including isoform 1 (316aa, 35kD), isoform 2 (287aa, 32.3kD) and isoform 3 (199aa, 22.2kD) . Rat sRANK-L has one isoform (318aa, 38.4kD) . 3963 can detect human, mouse and rat.

• Symbol:

  TNFSF11

• Positive Control 2:

  Cat. No. 1304 - Human Liver Tissue Lysate

• Positive Control 3:

  Cat. No. 10-201 - Human Liver Tissue Slide

• Positive Control 4:

  Cat. No. 1404 - Mouse Liver Tissue Lysate

• NCBI Official Name:

  Tumor necrosis factor (ligand) superfamily, member 11

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wong, et al. J. Biol. Chem. 1997; 272:25190-4.

• Background Reference 02:

  Kong et al. Nature 1999; 397:315-23.

• Background Reference 03:

  Weitzmann et al. Blood 2000; 96:1873-8.

• Background Reference 04:

  Bharti et al. J. Biol. Chem. 2004; 279:6065-76.

• Citation 01:

  PMID: 29226261.

• Other Product Names:

  SRANK Ligand Antibody: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  4507595

• Physical Properties:

  Liquid