BAG-1 Antibody

• Background:

  BAG-1 Antibody: Bcl-2-associated athanogene 1 (BAG-1) was first identified as an anti-apoptotic bcl-2-binding protein. Later it was found to bind the molecular chaperones Hsp70 and Hsc70 through its carboxy-terminal sequence (termed the Bag domain), resulting in the inhibition of the refolding activity of these chaperones. It is thought that by binding and inhibiting these molecular chaperones, BAG-1is able to modulate the expression level of proteins requiring chaperones to fold correctly. One such group of proteins that are affected is glucocorticoid receptors. Other reports have suggested that the level of BAG-1 expression correlates with the aggressiveness of various cancers. Multiple isoforms of BAG-1 are known to exist.

• NCBI Gene ID:

  573

• Swiss Prot:

  Q99933

• Accession Number:

  NP_004314

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cancer

• Purification:

  BAG-1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1216 - PC-3 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  BAG-1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  BAG-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  BAG1

• Positive Control 2:

  Cat. No. 1369 - Human Lymph node Tissue Lysate

• Positive Control 3:

  Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide

• NCBI Official Name:

  BCL2-associated athanogene

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Takayama S, Sato T, Kraweski K, et al. Cloning and functional analysis of BAG-1: a novel Bcl2-binding protein with anti-cell death activity. Cell 1995; 80:279-84.

• Background Reference 02:

  Nollen EAA, Brunsting JF, Song J, et al. Bag1 functions in vivo as a negative regulator of Hsp70 chaperone activity. Mol. Cell. Biol. 2000; 20:1083-8.

• Background Reference 03:

  Cato ACB and Mink S. BAG-1 family of cochaperones in the modulation of nuclear receptor action. J. Steroid Biochem. & Mol. Biol. 2001; 78:379-88.

• Background Reference 04:

  Kajewska M, Turner BC, Shabaik A, et al. Expression of BAG-1 protein correlates with aggressive behavior of prostate cancers. Prostate 2006; 66:801-10.

• Other Product Names:

  BAG-1 Antibody: HAP, BAG-1, RAP46, HAP, BAG family molecular chaperone regulator 1, Bcl-2-associated athanogene 1

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  124494251

• Physical Properties:

  Liquid