BAP31 Antibody

• Background:

  BAP31 Antibody: Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18. Bap31 may also play a role in the initiation of ER stress-induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria. Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis. The p20 fragment of Bap31, when expressed ectopically, is also a potent inducer cell death.

• NCBI Gene ID:

  10134

• Swiss Prot:

  P51572

• Accession Number:

  NP_005736

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  3675

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar BAP31 expression profile in human cell lines detected by two independent anti-BAP31 antibodies that recognize different epitopes, 3675 against central domain and 64-237, against recombinant protein. BAP31 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3675 64-237

• Field of Research:

  Signal Transduction

• Purification:

  BAP31 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1462 - Rat Lung Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  BAP31 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  BAP31 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human BAP31 has 2 isoforms, including isoform 1 (246aa, 28kD) and isoform 2 (313aa, 35kD) . Mouse BAP31 has one isoform (245aa, 28kD) and Rat BAP31 also has one isoform (245aa, 28kD) . 3675 can detect human, mouse and rat.

• Symbol:

  BCAP31

• Positive Control 2:

  Cat. No. 1204 - K562 Cell Lysate

• Positive Control 3:

  Cat. No. 1223 - Caco-2 Cell Lysate

• Positive Control 4:

  Cat. No. 1282 - 3T3/NIH Cell Lysate

• NCBI Official Name:

  B-cell receptor-associated protein 31

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ng et al. J. Cell Biol.1997; 139:327-38.

• Background Reference 02:

  Kim et al. EMBO J.1994; 13:3793-800.

• Background Reference 03:

  Zen et al. J. Biol. Chem.2004; 279:44924-30.

• Other Product Names:

  BAP31 Antibody: CDM, DDCH, BAP31, 6C6-AG, DXS1357E, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, BCR-associated protein 31

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  32171186

• Physical Properties:

  Liquid