Anthrax Lethal Factor Antibody

• Background:

  Anthrax Lethal Factor Antibody: Anthrax infection is initiated by the inhalation, ingestion, or cutaneous contact with Bacillus anthracis endospores. B. anthracis produces three polypeptides that comprise the anthrax toxin: protective antigen (PA), lethal factor (LF), and edema factor (EF) . PA binds to two related proteins on the cell surface; these are termed tumor epithelial marker 8 (TEM8) /anthrax toxin receptor (ATR) and capillary morphogenesis protein 2 (CMG2), although it is still unclear which is physiologically relevant. Following PA binding to its receptor, PA is cleaved into two fragments by a furin-like protease. The bound fragment binds both LF and EF; the resulting complex is then endocytosed which allows the translocation of LF and EF into the cytoplasm. LF is the primary toxin of anthrax and functions as a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family near their amino terminus, interfering with MAPK signaling and inducing apoptosis.

• NCBI Gene ID:

  3361711

• Swiss Prot:

  P15917

• Accession Number:

  P15917

• Host:

  Rabbit

• Reactivity:

  Bacteria

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Infectious Disease

• Purification:

  Anthrax Lethal Factor Antibody is affinity chromatography purified via peptide column.

• Concentration:

  1 mg/mL

• Buffer:

  Anthrax Lethal Factor Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Anthrax Lethal Factor antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Applications Notes:

  Anthrax lethal factor antibody can be used for the detection of Anthrax LF protein in ELISA. It will detect 10 ng of free peptide at 1 μ g/mL.

• Symbol:

  Pxo1_107

• NCBI Official Name:

  Lethal factor

• NCBI Organism:

  Bacillus anthracis

• Background Reference 01:

  Schwartz MN. Recognition and management of anthrax - an update. New Engl. J. Med. 2001; 345:1621-6.

• Background Reference 02:

  Moayeri M and Leppla SH. The roles of anthrax toxin in pathogenesis. Curr. Opin. Microbiol. 2004; 7:19-24.

• Background Reference 03:

  Bradley KA, Mogridge J, Mourez M, et al. Identification of the cellular receptor for anthrax toxin. Nature 2001; 414:225-9.

• Background Reference 04:

  Scobie HM, Rainey GJ, Bradley KA, et al. Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor. Proc. Natl. Acad. Sci. USA 2003; 100:5170-4.

• Other Product Names:

  Anthrax Lethal Factor Antibody: Lethal factor, Anthrax lethal toxin endopeptidase component, LF

• Tested Applications:

  ELISA

• Protein ID:

  50402185

• Physical Properties:

  Liquid