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Bim Antibody

CAT: 0223-3405-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-3405-01Size:0.02 mg
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Background
Bim Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
NCBI Gene ID
10018
Swiss Prot
O43521
Accession Number
O43521
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
3405
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar BIM expression profile in human and mouse cell lines detected by two independent anti-BIM antibodies that recognize different epitopes, 2065 against N-terminus domain and 3405 against central domain. BIM proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2065 3405 KD validation (Figure 7, 9, 10) : Anti-BIM antibody (3405) specificity was further verified by BIM specific knockdown. BIM signal in 293, U937, Jurkat and U266 cells transfected with BIM siRNAs or shRNA was disrupted in comparison with that in cells transfected with control siRNAs or shRNA. KD validation3405 Regulated expression validation (Figure 8) : BIM expression detected by anit-BIM antibodies (3405) was up-regulated by flavopiridol treatment, which was blocked by Cycloheximide in U266 cells. Regulated expression validation3405
Field of Research
Apoptosis, Cancer
Purification
Bim Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1204 - K562 Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Buffer
Bim Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Bim antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human BIM has 3 isoforms, including isoform EL (198aa, 22kD), isoform L (138aa, 16kD) and isoform S (108aa, 13kD) . Mouse BIM has 3 isoforms, including isoform EL (196aa, 22kD), isoform L (140aa, 16kD) and isoform S (110aa, 13kD) . Rat BIM has 4 isoforms, including isoform BOD-L (196aa, 22kD), isoform L (140aa, 16kD) and isoform BOD-M (110aa, 13kD) . 3405 only detect isoform EL.
Symbol
BCL2L11
Positive Control 2
Cat. No. 17-004 - K-562 Cell Slide
Positive Control 3
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 4
Cat. No. 1224 - Daudi Cell Lysate
NCBI Official Name
BCL2-like 11 (apoptosis facilitator)
NCBI Organism
Homo sapiens
Background Reference 01
O'Connor et al. EMBO J. 1998; 17:384-395.
Background Reference 02
Hsu et al. Mol. Endocrinol. 1998; 12:1432-40.
Citation 01
22693249
Citation 02
21198986
Citation 03
20404322
Citation 04
19805519
Citation 05
16081829
Citation 06
21148814
Citation 07
19234191
Citation 08
15014070
Citation 09
15713684
Citation 10
16478725
Citation 11
17374615
Citation 12
20388708
Citation 13
18094039
Citation 14
25002530
Citation 15
17067554
Citation 16
28599191
Other Product Names
Bim Antibody: BAM, BIM, BOD, Bcl-2-like protein 11, Bcl2-interacting mediator of cell death, Bcl2-L-11
Tested Applications
ELISA, WB, ICC, IF
Protein ID
18202042
Physical Properties
Liquid

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