MD-2 Antibody

• Background:

  MD-2 Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) . TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

• NCBI Gene ID:

  23643

• Swiss Prot:

  Q9Y6Y9

• Accession Number:

  NP_056179

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  3289

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Recombinant Protein Test (Figure 1) : Anti-MD-2 antibodies (3289) detected human MD-2 recombinant protein at different concentrations. Recombinant Protein Test3289

• Field of Research:

  Innate Immunity

• Purification:

  MD-2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1406 - Mouse Spleen Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  MD-2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  MD-2 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human MD-2 has 2 isoforms, including isoform 1 (160aa, 18kD) and isoform 2 (130aa, 15kD) . Mouse MD-2 has one isoform (160aa, 18kD) and Rat MD-2 also has one isoform (160aa, 18kD) . 3289 can detect human, mouse and rat.

• Symbol:

  LY96

• Positive Control 2:

  Cat. No. 1466 - Rat Spleen Tissue Lysate

• Positive Control 3:

  Cat. No. 10-901 - Human Spleen Tissue Slide

• NCBI Official Name:

  Lymphocyte antigen 96

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  O'Neill et al. Trends in Imm. 2003; 24:286-9.

• Background Reference 02:

  Vogel. Mol. Interv. 2003; 3:466-77.

• Background Reference 03:

  Takeda et al. Annu. Rev. Immunol. 2003; 21:335-76.

• Background Reference 04:

  Janeway et al. Annu. Rev. Immunol. 2002; 20:197-216.

• Other Product Names:

  MD-2 Antibody: MD2, MD-2, ly-96, ESOP-1, ESOP1, MD2, Lymphocyte antigen 96, Ly-96

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  223555998

• Physical Properties:

  Liquid