IRAK-4 Antibody

• Background:

  IRAK-4 Antibody: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR) . IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-κ B and MAP kinase (MAPK) by IL-1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4. Overexpression of IRAK-4 activates NF-κ B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

• NCBI Gene ID:

  51135

• Swiss Prot:

  Q9NWZ3

• Accession Number:

  AAM15772

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  3125

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar IRAK4 expression profile in human cell lines detected by two independent anti-IRAK4 antibodies that recognize different epitopes, 3125 against C-terminus domain and 24-025 against recombinant fusion protein. IRAK4 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3125 24-025 KO validation (Figure 6) : Anti-IRAK4 antibody (3125) specificity was further verified by IRAK4 knockout and mutant mice. IRAK4 signal in the macrophages of IRAK KO mice and mutant mice was disrupted as compared to wild type mice. KO validation3125 Regulated expression validation (Figure 7) : IRAK4 expression detected by anit-IRAK4 antibodies (3125) was reduced with CSK-4 treatment, which was rescued by proteasome inhibitors. Regulated expression validation3125 KD validation (Figure 8) : Anti-IRAK4 antibody (3125) specificity was further verified by IRAK4 knockdown. IRAK4 signal was disrupted in HEK293T cells transfected with IRAK4 siRNAs. KD validation3125

• Field of Research:

  Signal Transduction

• Purification:

  IRAK-4 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1204 - K562 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (85%), Mouse: (85%), Rat (77%)

• Buffer:

  IRAK-4 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  IRAK-4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  IRAK-4 antibody is predicted to not cross-react with other members of the IRAK protein family.

• Isoform:

  Human IRAK4 has 2 isoforms, including isoform 1 (460aa, 52kD) and isoform 2 (336aa, 38kD) . Mouse IRAK4 has one isoform (459aa, 51kD) and Rat IRAK4 also has one isoform (461aa, 51kD) . 3125 can detect human isoforms as well as mouse and rat IRAK4.

• Symbol:

  IRAK4

• Positive Control 2:

  Cat. No. 1201 - HeLa Cell Lysate

• Positive Control 3:

  Cat. No. 1224 -Daudi Cell Lysate

• Positive Control 4:

  Cat. No. 1211 -HepG2 Cell Lysate

• Positive Control 5:

  Cat. No. 17-004 - K-562 Cell Slide

• NCBI Official Name:

  Interleukin-1 receptor-associated kinase 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Cao et al. Science 1996; 271:1128-31

• Background Reference 02:

  Muzio et al. Science 1997; 278:1612-5.

• Background Reference 03:

  Wesche et al. J. Biol. Chem. 1999; 274:19403-10.

• Background Reference 04:

  Li et al. Proc. Natl. Acad. Sci. USA 2002; 99:5567-72.

• Citation 01:

  18266302

• Citation 02:

  15258191

• Citation 03:

  22158417

• Citation 04:

  17337443

• Other Product Names:

  IRAK-4 Antibody: IPD1, REN64, IRAK-4, NY-REN-64, Interleukin-1 receptor-associated kinase 4, Renal carcinoma antigen NY-REN-64

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  20219010

• Physical Properties:

  Liquid