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AIF Antibody

CAT: 0223-2239-01Size: 0.02 mgDry Ice: NoHazardous: No
CAT#:0223-2239-01Size:0.02 mg
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Background
AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) . Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed.
NCBI Gene ID
9131
Swiss Prot
O95381
Accession Number
O95381
Host
Rabbit
Reactivity
Human
Immunogen
2239
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar AIF expression profile in human and mouse cell lines detected by three independent anti-AIF antibodies that recognize different epitopes, 2267 against central domain, 2239 against N-terminus domain and 2301 against C-terminus domain. AIF proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies. Independent Antibody Validation in Cell lines2267 22392301 KD Validation (Figure 4,5) : Anti-AIF antibody specificity was further verified by AIF specific knockdown. AIF signal in H1299 cells and AIF stable cells transfected with AIF siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD Validation Localization Validation (Figure 7) : AIF detected by anti-AIF antibodies is colocalized with Mitotracker (mitochondrion marker) . AIF is localized in mitochondria. Localization Validation Induced Expression Validation (Figure 8) : AIF expression detected by anit-AIF antibodies was up-regulated by Magnolol treatment. Induced Expression Validation
Field of Research
Apoptosis, Cancer
Purification
AIF Antibody is Ion exchange chromatography purified.
Positive Control
Cat. No. 1204 - K562 Cell Lysate
Concentration
1 mg/ml
Homology
Predicted species reactivity based on immunogen sequence: Mouse: (50%), Rat: (50%)
Buffer
AIF Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
AIF antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Specificity
Multiple isoforms of AIF are known to exist.
Isoform
Human AIF has 6 isoforms, including isoform 1 (613aa, 67kD), isoform 2 (326aa, 36kD), isoform 3 (609aa, 66kD), isoform 4 (324aa, 35kD), isoform 5 (261aa, 28kD), and isoform 6 (237aa, 26kD) . Mouse AIF has one isoform (612aa, 67kD) and Rat AIF also has one isoform (612aa, 67kD) . 2239 can detect only 4 human isoforms (1,3,4,6) .
Symbol
AIFM1
Positive Control 2
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 3
Cat. No. 17-005 - Jurkat Cell Slide
Positive Control 4
Cat. No. 17-004 - K-562 Cell Slide
NCBI Official Name
Apoptosis inducing factor mitochondria associated 1
NCBI Organism
Homo sapiens
Background Reference 01
Zamzami et al. Nature 1999; 401:127-8.
Background Reference 02
Susin et al. Nature 1999; 397:441-6
Background Reference 03
Daugas et al. FASEB J. 2000; 14:729-39.
Citation 01
16729031
Citation 02
16195738
Citation 03
21937913
Citation 04
12939460
Citation 05
17142989
Citation 06
15619633
Citation 07
15131059
Citation 08
15126337
Citation 09
18519664
Citation 10
15297883
Citation 11
16645637
Citation 12
17553685
Citation 13
17980607
Citation 14
16273274
Citation 15
15699578
Citation 16
12140745
Citation 17
16454040
Citation 18
19414401
Citation 19
12140745
Other Product Names
AIF Antibody: Apoptosis-inducing factor 1, Programmed cell death protein 8, AIF, PDCD8
Tested Applications
ELISA, WB, IF
Protein ID
50400606
Physical Properties
Liquid

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