ICAD Antibody

• Background :

  ICAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which is cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.

• NCBI Gene ID :

  13347

• Swiss Prot :

  O54786

• Accession Number :

  O54786

• Host :

  Rabbit

• Reactivity :

  Mouse

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Apoptosis

• Purification :

  ICAD Antibody is affinity chromatography purified via peptide column.

• Positive Control :

  Cat. No. 1402 - Mouse Lung Tissue Lysate

• Concentration :

  1 mg/ml

• Buffer :

  ICAD Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  ICAD antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  45 kDa

• Fragment :

  IgG

• Symbol :

  Dffa

• NCBI Official Name :

  DNA fragmentation factor, alpha subunit

• NCBI Organism :

  Mus musculus

• Background Reference 01 :

  Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184

• Background Reference 02 :

  Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50

• Background Reference 03 :

  Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99

• Background Reference 04 :

  Wyllie A. Apoptosis. An endonuclease at last. Nature 1998;39120-21 (RD1299)

• Other Product Names :

  ICAD Antibody: ICAD, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, Icad, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45

• Tested Applications :

  ELISA, WB, IHC-P, IF

• Protein ID :

  9087146

• Physical Properties :

  Liquid