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CXCR4 Antibody

CAT: 0223-1009-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-1009-01Size:0.02 mg
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Background
CXCR4 signaling plays an important role in the migration, proliferation and quiescence of hematopoietic stem cell and their retention within the bone marrow, where it has high levels of SDF-1/CXCL12 (2) . It has been demonstrated that CXCR4 signaling mediates CD20 up-regulation on B cells (3) . CXCR4 is highly expressed in more than 23 types of cancer, including breast cancer, ovarian cancer, melanoma, and prostate cancer, while there is very less or no expression of CXCR4 in healthy tissues. CXCR4 expression in cancer cells has been reported to be associated with tumor survival, growth and metastasis in tissues with high levels of SDF-1/CXCL12, such as lungs, liver and bone marrow (4,5) . CXCR4 has been shown to regulate neuronal migration, cell positioning and axon wiring (6,7) . CXCR4 mutant mice displayed aberrant neuronal distribution, which implicates the role in neuronal disorders such as epilepsy. CXCR4 is also involved in WHIM syndrome (8) . WHIM mutations in CXCR4 were recently found in patients with Waldenstrom's macroglobulinemia, and these mutations are correlated to clinical resistance to ibrutinib (9,10) .
NCBI Gene ID
7852
Swiss Prot
P61073
Accession Number
NP_003458
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
1009
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation (Figure 2) shows similar CXCR4 expression profile in both human and mouse cell lines detected by two independent anti-CXCR4 antibodies that recognize different epitopes, 1009 against N-terminus ectodomain and 1012 against the second extracellular loop. CXCR4 proteins are detected in most of the cell lines, but not in THP-1 cells by the two independent antibodies. Additionally, Figure 2 shows the mouse CXCR4 protein in NIH/3T3 and C2C12 cell lines migrates slightly slower than human isoform b detected by both CXCR4 antibodies (1009 and 1012), which is well correlated with their calculated molecular masses (40.4 kDa vs 39.7 kDa) . Independent Antibody Validation1009 1012 siRNA knockdown validation (Figure 3) : Anti-CXCR4 antibody (1009) specificity was further verified by CXCR4 specific siRNA knockdown. CXCR4 signal in HeLa cells transfected with CXCR4 siRNAs was weaker in comparison with that in HeLa cells transfected with control siRNAs. siRNA knockdown validation1009 Animal Species Reactivity (Figure 4) : Anti-CXCR4 antibodies (1009 and 1012) can detect the expression of CXCR4 protein in rat thymus, but not in rat brain and rat heart. Animal Species Reactivity10091012 Recombinant Protein Test (Figure 5) shows that anti-CXCR4 antibody (1009) detects partial recombinant CXCR4 protein that contains the amino acids of 1009 antigenic peptide. A band with same size can be observed in coomassie blue staining. Recombinant Protein Test
Field of Research
Chemokines & Cytokines, Stem Cell, Infectious Disease
Purification
CXCR4 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1201 - HeLa Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (86%), Pig: (71%)
Buffer
CXCR4 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
CXCR4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Specificity
CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members.
Isoform
Human CXCR4 has four isoforms, including isoform a (356aa, 40.2kD), isoform b (352aa, 39.7kD), isoform c (423aa, 47.6kD), and isoform d (385aa, 43.4kD) . This antibody detects human isoform b only, but not other human isoforms. Mouse CXCR4 has two isoforms, including isoform 1 (359aa, 40.4kD) and isoform 2 (357aa, 40.2kD) . Rat CXCR4 has only one isoform identified so far (349aa, 39.4kD) .
Symbol
CXCR4
Positive Control 2
Cat. No. 1202 - A431 Cell Lysate
Positive Control 3
Cat. No. 1210 - 293 Cell Lysate
Positive Control 4
Cat. No. 1211 – HepG2 Cell Lysate
Positive Control 5
Cat. No. 1282 – NIH/3T3 Cell Lysate
Positive Control 6
Cat. No. 1471 – Rat thymus Lysate
NCBI Official Name
Chemokine (C-X-C motif) receptor 4
NCBI Organism
Homo sapiens
Background Reference 01
Dimitrov DS. Cell 1997;91:721-730
Background Reference 02
Chen et al. Circ Res. 2010;107:1083-93
Background Reference 03
Pavlasova et al. Blood 2016; 128: 1609-13
Background Reference 04
Sun et al. Cancer Metastasis Reviews 2010;29:709-22
Background Reference 05
Balkwill F. Nature Reviews 2004;4:540-50
Background Reference 06
Bagri et al. Development 2002; 129:4249-60
Background Reference 07
Yang et al. Development 2013;140:4554-64
Background Reference 08
Balabanjian et al. The Journal of Clinical Investigation 2008; 118:1074-84
Background Reference 09
Hunter et al. Blood 2014;123:1637-46
Background Reference 10
Treon et al. N. Engl. J. Med. 2015;372:1430-40
Citation 01
20197403
Citation 02
11799176
Citation 03
16638848
Citation 04
29954928
Citation 05
21309736
Citation 06
22039399
Citation 07
19525448
Citation 08
17515952
Citation 09
16825610
Other Product Names
CXCR4 Antibody: FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LESTR, NPY3R, NPYRL, HSY3RR, NPYY3R, D2S201E
Tested Applications
ELISA, WB, ICC, IF, Flow, IHC-P
Protein ID
4503175
Physical Properties
Liquid

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