Nuclease Serratia marcescens Recombinant Protein

• Expression System:

  E. coli

• Tag:

  His tag

• Endotoxin:

  < 0.5 EU/1000 units of the SuperNuclease as determined by the LAL method.

• Purity:

  > 95% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  The cleavage activity unit is defined as the amount of Ultra Nuclease that degrades DNA and causes 1 Absorbance Unit change at 260 nm (37°C for 30 minutes) which corresponds to complete digestion of 37 µg of DNA.

• Form:

  Liquid

• Buffer:

  20 mM Tris-HCl, 2 mM MgCl2, 2 mM NaCl, 50% glycerol, pH8.0.

• Molecular Weight:

  28 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Nuclease

• Species:

  Serratia marcescens

• Overview:

  A purified Ultra Nuclease also called Benzonase Nuclease, expressed in E.coli with demonstrated high endonuclease activity. Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany