Ifi35 (NM_027320) Mouse Recombinant Protein

• Background:

  Acts as a signaling pathway regulator involved in innate immune system response (PubMed:29350881) . In response to interferon IFN-alpha, associates in a complex with transcriptional regulator NMI to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35 and correlates with IFI35 dephosphorylation (By similarity) . In complex with NMI, inhibits virus-triggered type I interferon/IFN-beta production (By similarity) . In complex with NMI, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of the NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries (PubMed:29350881) . Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation when actively released by macrophage to the extracellular space during cell injury and pathogen invasion (By similarity) . Macrophage-secreted IFI35 activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  2010008K16Rik; AW986054; ifi-35; IFP35

• Gene ID:

  70110

• UniProt:

  Q9D8C4

• Accession Number mRNA:

  NM_027320

• Chromosomal Location:

  11 D

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  31.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ifi35

• Species:

  Mouse

• Protein ID:

  NP_081596

• Overview:

  Purified recombinant protein of Mouse interferon-induced protein 35 (Ifi35), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/70110

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9D8C4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_027320

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_081596