GIT2 (NM_139201) Human Recombinant Protein

• Background:

  This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008]

• Synonyms:

  CAT-2; CAT2; PKL

• Gene ID:

  9815

• UniProt:

  Q6FI58

• Accession Number mRNA:

  NM_139201

• Chromosomal Location:

  12q24.11

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Endocytosis

• Field of Research:

  Endocytosis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  52.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  GIT2

• Species:

  Human

• Protein ID:

  NP_631940

• Overview:

  Recombinant protein of human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 4

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/9815

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q6FI58

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_139201

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_631940