Lipoamide Dehydrogenase (DLD) (NM_000108) Human Recombinant Protein

• Background:

  This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

• Synonyms:

  DLDD; DLDH; E3; GCSL; LAD; OGDC-E3; PHE3

• Gene ID:

  1738

• UniProt:

  P09622, A0A024R713

• Accession Number mRNA:

  NM_000108

• Chromosomal Location:

  7q31.1

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Citrate cycle (TCA cycle), Glycine, serine and threonine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine degradation

• Field of Research:

  Citrate cycle (TCA cycle), Glycine, serine and threonine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine degradation

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  50.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  DLD

• Species:

  Human

• Protein ID:

  NP_000099

• Overview:

  Recombinant protein of human dihydrolipoamide dehydrogenase (DLD)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/1738

• Uniprot URL:

  https://www.uniprot.org/uniprot/P09622

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000108

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000099