Phosphoribosyl pyrophosphate amidotransferase (PPAT) (NM_002703) Human Recombinant Protein

• Background:

  The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]

• Synonyms:

  ATASE; GPAT; PRAT

• Gene ID:

  5471

• UniProt:

  Q06203, A8K4H7, Q59G63

• Accession Number mRNA:

  NM_002703

• Chromosomal Location:

  4q12

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism

• Field of Research:

  Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Protease

• Molecular Weight:

  57.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PPAT

• Species:

  Human

• Protein ID:

  NP_002694

• Overview:

  Recombinant protein of human phosphoribosyl pyrophosphate amidotransferase (PPAT)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/5471

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q06203

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_002703

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_002694