ATP6V1E1 (NM_001696) Human Recombinant Protein

• Background:

  This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]

• Synonyms:

  ARCL2C; ATP6E; ATP6E2; ATP6V1E; P31; Vma4

• Gene ID:

  529

• UniProt:

  P36543, Q53Y06

• Accession Number mRNA:

  NM_001696

• Chromosomal Location:

  22q11.21

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

• Field of Research:

  Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  26 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ATP6V1E1

• Species:

  Human

• Protein ID:

  NP_001687

• Overview:

  Recombinant protein of human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 (ATP6V1E1), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/529

• Uniprot URL:

  https://www.uniprot.org/uniprot/P36543

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001696

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001687