UMPS (NM_000373) Human Recombinant Protein

• Background:

  This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

• Synonyms:

  OPRT

• Gene ID:

  7372

• UniProt:

  P11172, A8K5J1

• Accession Number mRNA:

  NM_000373

• Chromosomal Location:

  3q21.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism

• Field of Research:

  Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  UMPS activity is verified in a bioassay:

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  52 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  UMPS

• Species:

  Human

• Protein ID:

  NP_000364

• Overview:

  Recombinant protein of human uridine monophosphate synthetase (UMPS)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/7372

• Uniprot URL:

  https://www.uniprot.org/uniprot/P11172

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000373

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000364