CoCoA (CALCOCO1) (NM_020898) Human Recombinant Protein

• Background:

  Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR) . Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1-mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions (By similarity) . In association with CCAR1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Calphoglin; Cocoa; PP13275

• Gene ID:

  57658

• UniProt:

  Q9P1Z2, A0A024RAZ1

• Accession Number mRNA:

  NM_020898

• Chromosomal Location:

  12q13.13

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Transcription Factors

• Molecular Weight:

  77.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CALCOCO1

• Species:

  Human

• Protein ID:

  NP_065949

• Overview:

  Recombinant protein of human calcium binding and coiled-coil domain 1 (CALCOCO1), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/57658

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9P1Z2

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_020898

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_065949