FZR1 (NM_016263) Human Recombinant Protein

• Background:

  Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:25349192) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  CDC20C; CDH1; FZR; FZR2; HCDH; HCDH1

• Gene ID:

  51343

• UniProt:

  Q9UM11

• Accession Number mRNA:

  NM_016263

• Chromosomal Location:

  19p13.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Cell cycle, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis

• Field of Research:

  Cell cycle, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  54.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FZR1

• Species:

  Human

• Protein ID:

  NP_057347

• Overview:

  Recombinant protein of human fizzy/cell division cycle 20 related 1 (Drosophila) (FZR1), transcript variant 2

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/51343

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9UM11

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016263

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_057347