EIF3s8 (EIF3C) (NM_001037808) Human Recombinant Protein

• Background:

  Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815) . The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632) . The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  EIF3-p110; EIF3CL; EIF3S8

• Gene ID:

  8663

• UniProt:

  Q99613, A0A024QYU9

• Accession Number mRNA:

  NM_001037808

• Chromosomal Location:

  16p11.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  105.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  EIF3C

• Species:

  Human

• Protein ID:

  NP_001032897

• Overview:

  Recombinant protein of human eukaryotic translation initiation factor 3, subunit C (EIF3C), transcript variant 2

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/8663

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q99613

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001037808

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001032897