SMAD4 (NM_005359) Human Recombinant Protein

• Background:

  This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF) -beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE) . The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Aug 2017]

• Synonyms:

  DPC4; JIP; MADH4; MYHRS

• Gene ID:

  4089

• UniProt:

  Q13485, A0A024R274

• Accession Number mRNA:

  NM_005359

• Chromosomal Location:

  18q21.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway

• Field of Research:

  Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  26772959

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Transcription Factors

• Molecular Weight:

  60.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SMAD4

• Species:

  Human

• Protein ID:

  NP_005350

• Overview:

  Recombinant protein of human SMAD family member 4 (SMAD4)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/4089

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q13485

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_005359

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_005350