VPS33A (NM_022916) Human Recombinant Protein

• Background:

  This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]

• Synonyms:

  MPSPS

• Gene ID:

  65082

• UniProt:

  Q96AX1

• Accession Number mRNA:

  NM_022916

• Chromosomal Location:

  12q24.31

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  67.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  VPS33A

• Species:

  Human

• Protein ID:

  NP_075067

• Overview:

  Recombinant protein of human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/65082

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q96AX1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_022916

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_075067