Insulin (INS) (NM_000207) Human Recombinant Protein

• Background:

  This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. [provided by RefSeq, May 2020]

• Synonyms:

  IDDM; IDDM1; IDDM2; ILPR; IRDN; MODY10; PNDM4

• Gene ID:

  3630

• UniProt:

  P01308, I3WAC9

• Accession Number mRNA:

  NM_000207

• Chromosomal Location:

  11p15.5

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Insulin signaling pathway, Maturity onset diabetes of the young, mTOR signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Regulation of autophagy, Type I diabetes mellitus, Type II diabetes mellitus

• Field of Research:

  Insulin signaling pathway, Maturity onset diabetes of the young, mTOR signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Regulation of autophagy, Type I diabetes mellitus, Type II diabetes mellitus

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  28417915

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein

• Molecular Weight:

  9.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  INS

• Species:

  Human

• Protein ID:

  NP_000198

• Overview:

  Recombinant protein of human insulin (INS)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/3630

• Uniprot URL:

  https://www.uniprot.org/uniprot/P01308

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000207

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000198