Apc5 (ANAPC5) (NM_016237) Human Recombinant Protein

• Background:

  This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]

• Synonyms:

  APC5

• Gene ID:

  51433

• UniProt:

  Q9UJX4

• Accession Number mRNA:

  NM_016237

• Chromosomal Location:

  12q24.31

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis

• Field of Research:

  Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  84.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ANAPC5

• Species:

  Human

• Protein ID:

  NP_057321

• Overview:

  Recombinant protein of human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/51433

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9UJX4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016237

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_057321