SPHK1 (NM_021972) Human Recombinant Protein

• Background:

  The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]

• Synonyms:

  SPHK

• Gene ID:

  8877

• UniProt:

  Q9NYA1

• Accession Number mRNA:

  NM_021972

• Chromosomal Location:

  17q25.1

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway

• Field of Research:

  Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  43.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SPHK1

• Species:

  Human

• Protein ID:

  NP_068807

• Overview:

  Recombinant protein of human sphingosine kinase 1 (SPHK1), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/8877

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9NYA1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_021972

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_068807