Stefin B (CSTB) (NM_000100) Human Recombinant Protein

• Background:

  The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1) . One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies. [provided by RefSeq, Jul 2016]

• Synonyms:

  CPI-B; CST6; EPM1; EPM1A; PME; STFB; ULD

• Gene ID:

  1476

• UniProt:

  P04080, Q76LA1

• Accession Number mRNA:

  NM_000100

• Chromosomal Location:

  21q22.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  11 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CSTB

• Species:

  Human

• Protein ID:

  NP_000091

• Overview:

  Recombinant protein of human cystatin B (stefin B) (CSTB)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/1476

• Uniprot URL:

  https://www.uniprot.org/uniprot/P04080

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000100

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000091