SMAD2 (NM_005901) Human Recombinant Protein

• Background:

  The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF) -beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

• Synonyms:

  HMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2

• Gene ID:

  4087

• UniProt:

  Q15796, Q53XR6

• Accession Number mRNA:

  NM_005901

• Chromosomal Location:

  18q21.1

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Adherens junction, Cell cycle, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway

• Field of Research:

  Adherens junction, Cell cycle, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  26772959

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors

• Molecular Weight:

  52.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SMAD2

• Species:

  Human

• Protein ID:

  NP_005892

• Overview:

  Recombinant protein of human SMAD family member 2 (SMAD2), transcript variant 1

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/4087

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q15796

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_005901

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_005892