PRAF1 (POLR1E) (NM_022490) Human Recombinant Protein

• Background:

  DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  A49; PAF53; PRAF1; RPA49

• Gene ID:

  64425

• UniProt:

  Q9GZS1

• Accession Number mRNA:

  NM_022490

• Chromosomal Location:

  9p13.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

• Field of Research:

  Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Transcription Factors

• Molecular Weight:

  47.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  POLR1E

• Species:

  Human

• Protein ID:

  NP_071935

• Overview:

  Recombinant protein of human polymerase (RNA) I polypeptide E, 53kDa (POLR1E)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/64425

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9GZS1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_022490

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_071935