Osteopontin (SPP1) (NM_000582) Human Recombinant Protein

• Background:

  The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

• Synonyms:

  BNSP; BSPI; ETA-1; OPN

• Gene ID:

  6696

• UniProt:

  P10451, A0A024RDE6

• Accession Number mRNA:

  NM_000582

• Chromosomal Location:

  4q22.1

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway

• Field of Research:

  ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  29959420

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome, Secreted Protein

• Molecular Weight:

  32.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SPP1

• Species:

  Human

• Protein ID:

  NP_000573

• Overview:

  Purified recombinant protein of Homo sapiens secreted phosphoprotein 1 (SPP1), transcript variant 2

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/6696

• Uniprot URL:

  https://www.uniprot.org/uniprot/P10451

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000582

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000573