UBE2D1 (NM_003338) Human Recombinant Protein

• Background:

  The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

• Synonyms:

  E2 (17) KB1; SFT; UBC4/5; UBCH5; UBCH5A

• Gene ID:

  7321

• UniProt:

  P51668, A0A024QZJ2

• Accession Number mRNA:

  NM_003338

• Chromosomal Location:

  10q21.1

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Ubiquitin mediated proteolysis

• Field of Research:

  Ubiquitin mediated proteolysis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  16.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  UBE2D1

• Species:

  Human

• Protein ID:

  NP_003329

• Overview:

  Recombinant protein of human ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast) (UBE2D1)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/7321

• Uniprot URL:

  https://www.uniprot.org/uniprot/P51668

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_003338

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_003329