ECH1 (NM_001398) Human Recombinant Protein

• Background:

  This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]

• Synonyms:

  HPXEL

• Gene ID:

  1891

• UniProt:

  Q13011, A0A384MR44

• Accession Number mRNA:

  NM_001398

• Chromosomal Location:

  19q13.2

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Molecular Weight:

  35.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ECH1

• Species:

  Human

• Protein ID:

  NP_001389

• Overview:

  Recombinant protein of human enoyl Coenzyme A hydratase 1, peroxisomal (ECH1)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/1891

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q13011

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001398

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001389